DO sensors can be designed based on different technological principles, with one of them being the fluorescence method. Fluorescence-based dissolved oxygen sensors utilize a specific material’s response to active fluorescence extinction, a principle from physics. Typically, the sensor’s front end consists of two light-emitting diodes (LEDs), one emitting blue light and the other emitting red light, along with a photodetector and a fluorescence cap.

The blue LED emits modulated blue light, which irradiates the fluorescent material on the inner surface of the cap, causing it to emit red fluorescence upon excitation. The photodetector detects the red fluorescence and compares it with a reference light. By calculating the phase difference between the two and comparing it with internal calibration values, the concentration of oxygen molecules can be determined. Additionally, the sensor automatically compensates for temperature and pressure to output the final dissolved oxygen concentration value.

This fluorescence-based DO sensor has a simple structure and leverages the properties of fluorescent materials to achieve precise dissolved oxygen concentration measurements, providing reliable data in various environmental conditions.

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DO Sensor FAQs

Electrochemical DO sensors, also known as amperometric or Clark-type sensors, measure dissolved oxygen concentration in water based on electrical current produced. Polarographic and galvanic are types of electrochemical DO sensors.

There are two types of electrochemical DO sensors: galvanic and polarographic. Polarographic dissolved oxygen sensors can be further broken down into steady-state and rapid-pulsing sensors. Both galvanic and polarographic DO sensors use two polarized electrodes, an anode and a cathode, in an electrolyte solution 7.
 

Dissolved oxygen probes work by measuring the amount of oxygen that diffuses across a membrane into the sensor. Once oxygen is inside the sensor, a chemical reduction reaction occurs, producing an electrical signal. This signal is read by the DO probe and is displayed on a meter.

Dissolved oxygen (DO) sensors perform liquid process measurement for determining the amount of oxygen dissolved or carried in the process liquid. There are three common technologies for dissolved oxygen measurements: polarographic, galvanic and optical.

Dissolved oxygen measurement range is 0~20mg/L (0~200% saturation).
 
Optical sensors provide a high degree of accuracy from 0% to 100% DO. They are especially useful at low DO concentrations and are one of the best options for tissue culture where oxygen measurement is extremely important.
 

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